Solid-state NMR spectroscopy applied to a chimeric potassium channel in lipid bilayers
Journal of the American Chemical Society, ISSN: 0002-7863, Vol: 130, Issue: 23, Page: 7427-7435
2008
- 68Citations
- 79Captures
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Metrics Details
- Citations68
- Citation Indexes68
- CrossRef68
- 66
- Captures79
- Readers79
- 79
Article Description
We show that solid-state NMR can be used to investigate the structure and dynamics of a chimeric potassium channel, KcsA-Kv1.3, in lipid bilayers. Sequential resonance assignments were obtained using a combination of N-C and C-C correlation experiments conducted on fully labeled and reverse-labeled as well as C-terminally truncated samples. Comparison of our results with those from X-ray crystallography and solution-state NMR in micelles on the closely related KcsA K channel provides insight into the mechanism of ion channel selectivity and underlines the important role of the lipid environment for membrane protein structure and function. © 2008 American Chemical Society.
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