Two-photon excitation spectroscopy of carotenoid-containing and carotenoid-depleted LH2 complexes from purple bacteria
Journal of Physical Chemistry B, ISSN: 1520-6106, Vol: 113, Issue: 34, Page: 11720-11723
2009
- 21Citations
- 23Captures
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Metrics Details
- Citations21
- Citation Indexes21
- 21
- CrossRef19
- Captures23
- Readers23
- 23
Article Description
We applied two-photon fluorescence excitation spectroscopy to LH2 complex from purple bacteria Allochromatium minutissimum and Rhodobacter sphaeroides. Bacteriochlorophyll fluorescence was measured under two-photon excitation of the samples within the 1200-1500 nm region. Spectra were obtained for both carotenoid-containing and -depleted complexes of each bacterium to allow their direct comparison. The depletion of carotenoids did not alter the two-photon excitation spectra of either bacteria. The spectra featured a wide excitation band around 1350 nm (2 × 675 nm, 14 800 cm) which strongly resembled two-photon fluorescence excitation spectra of similar complexes published by other authors. We consider obtained experimental data to be evidence of direct two-photon excitation of bacteriochlorophyll excitonic states in this spectral region. © 2009 American Chemical Society.
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