Simultaneous Determination of Unlabeled and Deuterium-Labeled Indinavir in Human Plasma by High-Performance Liquid Chromatography with Tandem Mass Spectrometric Detection
Journal of Pharmaceutical Sciences, ISSN: 0022-3549, Vol: 86, Issue: 2, Page: 193-198
1997
- 36Citations
- 6Captures
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Article Description
A method for the simultaneous determination of indinavir and its hexadeuterated analog (d 6 -indinavir) in human plasma is described. Isolation of the analytes and internal standard from plasma was achieved via liquid-liquid extraction with methyl- t -butyl ether. The analytes were chromatographed under reversed-phase conditions on a BDS-Hypersil C8 column. A Sciex API III+ tandem mass spectrometer equipped with a turbo ion-spray interface was used as the detector. Multiple reaction monitoring using the parent→product ion combinations of m/z 614→465, 620→471 and 654→505 were used to quantify indinavir, d 6 -indinavir, and internal standard, respectively. The method was validated, using 1-mL aliquots of plasma, in the concentration range in plasma of 1 to 200 ng/mL. Precision of the assay, as measured by the coefficient of variation, ranged from 0.9 to 4.3% and 0.9 to 6.2% for indinavir and d 6 -indinavir, respectively. Indinavir assay accuracy ranged from 95.8 to 105.0% of nominal, whereas the accuracy of the assay for d 6 -indinavir ranged from 97.4 to 104.0% of nominal. The assay was used to support a clinical study in which the stable isotope technique was used to determine the bioavailability of indinavir.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0022354915502451; http://dx.doi.org/10.1021/js960288j; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0031046894&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/9040094; http://linkinghub.elsevier.com/retrieve/pii/S0022354915502451; https://dx.doi.org/10.1021/js960288j
American Geophysical Union (AGU)
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