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Unraveling the primary isomerization dynamics in cyanobacterial phytochrome Cph1 with multipulse manipulations

Journal of Physical Chemistry Letters, ISSN: 1948-7185, Vol: 4, Issue: 16, Page: 2605-2609
2013
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Article Description

The ultrafast mechanisms underlying the initial photoisomerization (P → Lumi-R) in the forward reaction of the cyanobacterial photoreceptor Cph1 were explored with multipulse pump-dump-probe transient spectroscopy. A recently postulated multipopulation model was used to fit the transient pump-dump-probe and dump-induced depletion signals. We observed dump-induced depletion of the Lumi-R photoproduct, demonstrating that photoisomerization occurs via evolution on both the excited- and ground-state electronic surfaces. Excited-state equilibrium was not observed, as shown via the absence of a dump-induced excited-state "Le Chaîtelier redistribution" of excited-state populations. The importance of incorporating the inhomogeneous dynamics of Cph1 in interpreting measured transient data is discussed. © 2013 American Chemical Society.

Bibliographic Details

Kim, Peter W; Rockwell, Nathan C; Freer, Lucy H; Chang, Che-Wei; Martin, Shelley S; Lagarias, J Clark; Larsen, Delmar S

American Chemical Society (ACS)

Materials Science; Chemistry

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