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AMPLIFIED multiplexed analysis of DNA by the exonuclease iii-catalyzed regeneration of the target dna in the presence of functionalized semiconductor quantum dots

Nano Letters, ISSN: 1530-6984, Vol: 11, Issue: 10, Page: 4456-4461
2011
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Article Description

Quantum dots (QDs) functionalized with a black-hole quencher are used as optical tracer for the detection of DNA using exonuclease as a biocatalyst. The binding of the target DNA or of a target/open hairpin complex to the functionalized QDs leads to the exonuclease-stimulated recycling of the target DNA or the target/hairpin complex. This results in the triggering of the luminescence of the QDs that provides a readout signal for the amplified sensing process. By using different-sized QDs, the multiplexed detection of DNAs is demonstrated. © 2011 American Chemical Society.

Bibliographic Details

Freeman, Ronit; Liu, Xiaoqing; Willner, Itamar

American Chemical Society (ACS)

Chemical Engineering; Chemistry; Materials Science; Physics and Astronomy; Engineering

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