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Genetic analysis of chemoresistance in primary murine lymphomas

Nature Medicine, ISSN: 1078-8956, Vol: 6, Issue: 9, Page: 1029-1035
2000
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Article Description

Understanding the basis of chemoresistance is a principal goal of molecular oncology. We have exploited a murine lymphoma model and retroviral gene transfer to rapidly generate a series of spontaneous tumors differing only in a gene of interest, and subsequently studied the impact of the test gene on the treatment sensitivity of tumors at their natural site. We demonstrate that the Bcl-2 oncoprotein produces multi-drug resistance when assessed in primary lymphomas in vivo. In contrast, this effect was dramatically reduced when the primary lymphomas were subjected to long-term culture, and completely missed in the standard clonogenic survival assay. This model highlights the importance of physiological test systems to address the complexity of clinical drug resistance and provides a novel strategy to evaluate compounds targeting specific genetic lesions.

Bibliographic Details

Clemens A. Schmitt; Christine T. Rosenthal; Scott W. Lowe

Springer Science and Business Media LLC

Biochemistry, Genetics and Molecular Biology

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