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The etiologic role of human papillomavirus in penile cancers: A study in Vietnam

British Journal of Cancer, ISSN: 0007-0920, Vol: 108, Issue: 1, Page: 229-233
2013
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Background:We investigated the aetiologic role of human papillomavirus (HPV) in 120 penile squamous cell carcinomas (PSCCs) from Vietnam.Methods:Human papillomavirus DNA was detected by PCR using SPF10 primers and a primer set targeting HPV-16 E6. The INNO-LiPA HPV genotyping kit was used to determine genotype. Human papillomavirus-16 viral load and physical status were determined by real-time PCR. P16 INK4A protein expression was investigated by immunohistochemistry.Results:Human papillomavirus DNA was detected in 27 of 120 (23%) PSCCs. The most frequently detected genotype was HPV-16 (24 of 27 cases, 89%). In 16 of 18 (89%) HPV-16-positive cases, the HPV DNA was considered to be integrated into the host genome. The geometric mean of the HPV-16 viral load was 0.4 copies per cell. P16 INK4A overexpression was significantly related to PSCCs infected with high-risk HPV (P=0.018) and HPV-16 copy numbers (P<0.001).Conclusion:Human papillomavirus-16 DNA integration and p16 INK4A overexpression in high-risk HPV detected PSCCs suggested an aetiologic role of high-risk HPV in the development of PSCCs. © 2013 Cancer Research UK. All rights reserved.

Bibliographic Details

H. T.T. Do; C. Koriyama; N. A. Khan; T. Kato; S. Akiba; M. Higashi; N. T. Le; S. Matsushita; T. Kanekura

Springer Science and Business Media LLC

Medicine; Biochemistry, Genetics and Molecular Biology

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