High-level direct expression of semi-synthetic human interiiukin-6 in escherichia couand production of n-terminus met-free product
Bio/Technology, ISSN: 0733-222X, Vol: 8, Issue: 11, Page: 1036-1040
1990
- 44Citations
- 1Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations44
- Citation Indexes44
- 44
- CrossRef17
- Captures1
- Readers1
Article Description
We have developed a direct expression system for high-level production of recombinant human interleukin-6 (rhIL-6) in Escherichia coli. In this system, (i) the natural N-terminal coding region of the hIL-6 gene was replaced by a synthetic sequence containing A-T rich codons, (ii) dual Shine-Dalgarno (SD) sequences were employed, (iii) an A-T rich segment was inserted in front of the initiation codon to avoid putative mRNA secondary structure in the region and (iv) the natural amber termination codon of the hIL-6 gene was changed to an ocher stop codon. The hIL-6 polypeptide, synthesized at a high level, formed cytoplasmic inclusion bodies. After refolding, the N-terminal methionine was removed by aminopeptidase-P in vitro. The purified recombinant hIL-6 had B-cell differentiation activity equivalent to natural IL-6 from a human T-cell culture.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=14744276967&origin=inward; http://dx.doi.org/10.1038/nbt1190-1036; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0025225635&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/1366931; https://www.nature.com/doifinder/10.1038/nbt1190-1036; https://dx.doi.org/10.1038/nbt1190-1036; https://www.nature.com/articles/nbt1190-1036
Springer Science and Business Media LLC
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