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Overlap between folding and functional energy landscapes for adenylate kinase conformational change

Nature Communications, ISSN: 2041-1723, Vol: 1, Issue: 8, Page: 111
2010
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Article Description

Enzyme function is often dependent on fluctuations between inactive and active structural ensembles. Adenylate kinase isolated from Escherichia coli (AK) is a small phosphotransfer enzyme in which interconversion between inactive (open) and active (closed) conformations is rate limiting for catalysis. AK has a modular three-dimensional architecture with two flexible substrate-binding domains that interact with the substrates AMP, ADP and ATP. Here, we show by using a combination of biophysical and mutagenic approaches that the interconversion between open and closed states of the ATP-binding subdomain involves partial subdomain unfolding/refolding in an otherwise folded enzyme. These results provide a novel and, possibly general, molecular mechanism for the switch between open and closed conformations in AK. © 2010 Macmillan Publishers Limited. All rights reserved.

Bibliographic Details

Olsson, Ulrika; Wolf-Watz, Magnus

Springer Science and Business Media LLC

Chemistry; Biochemistry, Genetics and Molecular Biology; Physics and Astronomy

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