Mechanical memory and dosing influence stem cell fate
Nature Materials, ISSN: 1476-4660, Vol: 13, Issue: 6, Page: 645-652
2014
- 868Citations
- 1,229Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations868
- Citation Indexes867
- 867
- CrossRef710
- Patent Family Citations1
- Patent Families1
- Captures1,229
- Readers1,229
- 1,226
Article Description
We investigated whether stem cells remember past physical signals and whether these can be exploited to dose cells mechanically. We found that the activation of the Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding domain (TAZ) as well as the pre-osteogenic transcription factor RUNX2 in human mesenchymal stem cells (hMSCs) cultured on soft poly(ethylene glycol) (PEG) hydrogels (Young's modulus E ∼ 2 kPa) depended on previous culture time on stiff tissue culture polystyrene (TCPS; E ∼ 3 GPa). In addition, mechanical dosing of hMSCs cultured on initially stiff (E ∼ 10 kPa) and then soft (E ∼ 2 kPa) phototunable PEG hydrogels resulted in either reversible or-above a threshold mechanical dose-irreversible activation of YAP/TAZ and RUNX2. We also found that increased mechanical dosing on supraphysiologically stiff TCPS biases hMSCs towards osteogenic differentiation. We conclude that stem cells possess mechanical memory-with YAP/TAZ acting as an intracellular mechanical rheostat-that stores information from past physical environments and influences the cells fate. © 2014 Macmillan Publishers Limited.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84901232687&origin=inward; http://dx.doi.org/10.1038/nmat3889; http://www.ncbi.nlm.nih.gov/pubmed/24633344; https://facultyopinions.com/prime/718313506#eval793504448; http://dx.doi.org/10.3410/f.718313506.793504448; https://www.nature.com/articles/nmat3889; https://facultyopinions.com/prime/718313506#eval793492429; http://dx.doi.org/10.3410/f.718313506.793492429; https://dx.doi.org/10.1038/nmat3889; http://f1000.com/prime/718313506#eval793492429
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