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MGRASP enables mapping mammalian synaptic connectivity with light microscopy

Nature Methods, ISSN: 1548-7105, Vol: 9, Issue: 1, Page: 96-102
2012
  • 222
    Citations
  • 0
    Usage
  • 848
    Captures
  • 1
    Mentions
  • 1
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    222
  • Captures
    848
  • Mentions
    1
    • Blog Mentions
      1
      • Blog
        1
  • Social Media
    1
    • Shares, Likes & Comments
      1
      • Facebook
        1

Article Description

The GFP reconstitution across synaptic partners (GRASP) technique, based on functional complementation between two nonfluorescent GFP fragments, can be used to detect the location of synapses quickly, accurately and with high spatial resolution. The method has been previously applied in the nematode and the fruit fly but requires substantial modification for use in the mammalian brain. We developed mammalian GRASP (mGRASP) by optimizing transmembrane split-GFP carriers for mammalian synapses. Using in silico protein design, we engineered chimeric synaptic mGRASP fragments that were efficiently delivered to synaptic locations and reconstituted GFP fluorescence in vivo. Furthermore, by integrating molecular and cellular approaches with a computational strategy for the three-dimensional reconstruction of neurons, we applied mGRASP to both long-range circuits and local microcircuits in the mouse hippocampus and thalamocortical regions, analyzing synaptic distribution in single neurons and in dendritic compartments. © 2012 Nature America, Inc. All rights reserved.

Bibliographic Details

Kim, Jinhyun; Zhao, Ting; Petralia, Ronald S; Yu, Yang; Peng, Hanchuan; Myers, Eugene; Magee, Jeffrey C

Springer Science and Business Media LLC

Biochemistry, Genetics and Molecular Biology

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