Mass spectrometry-based detection and quantification of plasma glycoproteins using selective reaction monitoring
Nature Protocols, ISSN: 1750-2799, Vol: 7, Issue: 5, Page: 859-871
2012
- 30Citations
- 66Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations30
- Citation Indexes30
- 30
- CrossRef27
- Captures66
- Readers66
- 66
Article Description
Mass spectrometry-based targeted proteomics is a rapidly expanding method for quantifying proteins in complex clinical samples such as plasma. In conjunction with the stable isotope dilution method, selected reaction monitoring (srM) assays provide unparalleled sensitivity and selectivity for detection and quantification. acrucial factor for robust srM assays is the reduction of interference by lowering the background. this can be achieved by the selective isolation of a subproteome, such as N-glycosylated proteins, from the original sample. the present protocol includes the development and optimization of srM assays associated with each peptide of interest and the qualification of assays in the biological matrix to establish the limits of detection and quantification. the protocol also describes the enrichment of formerly N-glycosylated peptides relying on periodate oxidation of glycan moieties attached to the proteins, their immobilization on solid supports through hydrazide chemistry, proteolysis and enzymatic release of the formerly N-glycosylated peptides. © 2012 Nature America, Inc.
Bibliographic Details
Springer Science and Business Media LLC
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