Preparation of viable adult ventricular myocardial slices from large and small mammals
Nature Protocols, ISSN: 1750-2799, Vol: 12, Issue: 12, Page: 2623-2639
2017
- 80Citations
- 235Captures
- 2Mentions
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations80
- Citation Indexes80
- 80
- CrossRef31
- Captures235
- Readers235
- 235
- Mentions2
- News Mentions2
- 2
Most Recent News
How to prepare and assess Living Myocardial Slices – Workshop Imperial Centre for Translational and Experimental Medicine (ICTEM), Hammersmith Campus
The Workshop will provide participants with all the necessary knowledge and practical skill to implement this technique in their own laboratories. Living Myocardial Slices (LMS)
Article Description
T his protocol describes the preparation of highly viable adult ventricular myocardial slices from the hearts of small and large mammals, including rodents, pigs, dogs and humans. Adult ventricular myocardial slices are 100- to 400-mm-thick slices of living myocardium that retain the native multicellularity, architecture and physiology of the heart. This protocol provides a list of the equipment and reagents required alongside a detailed description of the methodology for heart explantation, tissue preparation, slicing with a vibratome and handling of myocardial slices. Supplementary videos are included to visually demonstrate these steps. A number of critical steps are addressed that must be followed in order to prepare highly viable myocardial slices. These include identification of myocardial fiber direction and fiber alignment within the tissue block, careful temperature control, use of an excitationcontraction uncoupler, optimal vibratome settings and correct handling of myocardial slices. Many aspects of cardiac structure and function can be studied using myocardial slices in vitro. Typical results obtained with hearts from a small mammal (rat) and a large mammal (human) with heart failure are shown, demonstrating myocardial slice viability, maximum contractility, Ca2+ handling and structure. This protocol can be completed in 4 h.
Bibliographic Details
Springer Science and Business Media LLC
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