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Binding of HMGN proteins to cell specific enhancers stabilizes cell identity

Nature Communications, ISSN: 2041-1723, Vol: 9, Issue: 1, Page: 5240
2018
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Article Description

The dynamic nature of the chromatin epigenetic landscape plays a key role in the establishment and maintenance of cell identity, yet the factors that affect the dynamics of the epigenome are not fully known. Here we find that the ubiquitous nucleosome binding proteins HMGN1 and HMGN2 preferentially colocalize with epigenetic marks of active chromatin, and with cell-type specific enhancers. Loss of HMGNs enhances the rate of OSKM induced reprogramming of mouse embryonic fibroblasts (MEFs) into induced pluripotent stem cells (iPSCs), and the ASCL1 induced conversion of fibroblast into neurons. During transcription factor induced reprogramming to pluripotency, loss of HMGNs accelerates the erasure of the MEF-specific epigenetic landscape and the establishment of an iPSCs-specific chromatin landscape, without affecting the pluripotency potential and the differentiation potential of the reprogrammed cells. Thus, HMGN proteins modulate the plasticity of the chromatin epigenetic landscape thereby stabilizing, rather than determining cell identity.

Bibliographic Details

He, Bing; Deng, Tao; Zhu, Iris; Furusawa, Takashi; Zhang, Shaofei; Tang, Wei; Postnikov, Yuri; Ambs, Stefan; Li, Caiyi Cherry; Livak, Ferenc; Landsman, David; Bustin, Michael

Springer Science and Business Media LLC

Chemistry; Biochemistry, Genetics and Molecular Biology; Physics and Astronomy

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