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Exploring beyond clinical routine SARS-CoV-2 serology using MultiCoV-Ab to evaluate endemic coronavirus cross-reactivity

Nature Communications, ISSN: 2041-1723, Vol: 12, Issue: 1, Page: 1152
2021
  • 57
    Citations
  • 0
    Usage
  • 110
    Captures
  • 3
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    57
  • Captures
    110
  • Mentions
    3
    • References
      2
      • Wikipedia
        2
    • News Mentions
      1
      • News
        1

Most Recent News

Study shows both importance of second vaccine dose and updating vaccines to combat new variants of concern

New research presented at this year's European Congress of Clinical Microbiology & Infectious Diseases (ECCMID) shows the importance of receiving the second dose of a COVID-19 vaccine and also the need to constantly review and update vaccines to deal with new variants of concern. The study is by Dr. Nicole Schneiderhan-Marra, Natural and Medical Sciences Institute at the University of Tübingen, Re

Article Description

The humoral immune response to SARS-CoV-2 is a benchmark for immunity and detailed analysis is required to understand the manifestation and progression of COVID-19, monitor seroconversion within the general population, and support vaccine development. The majority of currently available commercial serological assays only quantify the SARS-CoV-2 antibody response against individual antigens, limiting our understanding of the immune response. To overcome this, we have developed a multiplex immunoassay (MultiCoV-Ab) including spike and nucleocapsid proteins of SARS-CoV-2 and the endemic human coronaviruses. Compared to three broadly used commercial in vitro diagnostic tests, our MultiCoV-Ab achieves a higher sensitivity and specificity when analyzing a well-characterized sample set of SARS-CoV-2 infected and uninfected individuals. We find a high response against endemic coronaviruses in our sample set, but no consistent cross-reactive IgG response patterns against SARS-CoV-2. Here we show a robust, high-content-enabled, antigen-saving multiplex assay suited to both monitoring vaccination studies and facilitating epidemiologic screenings for humoral immunity towards pandemic and endemic coronaviruses.

Bibliographic Details

Becker, Matthias; Strengert, Monika; Junker, Daniel; Kaiser, Philipp D; Kerrinnes, Tobias; Traenkle, Bjoern; Dinter, Heiko; Häring, Julia; Ghozzi, Stéphane; Zeck, Anne; Weise, Frank; Peter, Andreas; Hörber, Sebastian; Fink, Simon; Ruoff, Felix; Dulovic, Alex; Bakchoul, Tamam; Baillot, Armin; Lohse, Stefan; Cornberg, Markus; Illig, Thomas; Gottlieb, Jens; Smola, Sigrun; Karch, André; Berger, Klaus; Rammensee, Hans-Georg; Schenke-Layland, Katja; Nelde, Annika; Märklin, Melanie; Heitmann, Jonas S; Walz, Juliane S; Templin, Markus; Joos, Thomas O; Rothbauer, Ulrich; Krause, Gérard; Schneiderhan-Marra, Nicole

Springer Science and Business Media LLC

Chemistry; Biochemistry, Genetics and Molecular Biology; Physics and Astronomy

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