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Ribosome collisions induce mRNA cleavage and ribosome rescue in bacteria

Nature, ISSN: 1476-4687, Vol: 603, Issue: 7901, Page: 503-508
2022
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Bacterial ribosomes: molecular collision activates protection mechanism

Ribosomes are vitally important for all living organisms. They translate the information stored in the messenger molecule mRNA into proteins. To do this, they bind

Article Description

Ribosome rescue pathways recycle stalled ribosomes and target problematic mRNAs and aborted proteins for degradation. In bacteria, it remains unclear how rescue pathways distinguish ribosomes stalled in the middle of a transcript from actively translating ribosomes. Here, using a genetic screen in Escherichia coli, we discovered a new rescue factor that has endonuclease activity. SmrB cleaves mRNAs upstream of stalled ribosomes, allowing the ribosome rescue factor tmRNA (which acts on truncated mRNAs) to rescue upstream ribosomes. SmrB is recruited to ribosomes and is activated by collisions. Cryo-electron microscopy structures of collided disomes from E. coli and Bacillus subtilis show distinct and conserved arrangements of individual ribosomes and the composite SmrB-binding site. These findings reveal the underlying mechanisms by which ribosome collisions trigger ribosome rescue in bacteria.

Bibliographic Details

Saito, Kazuki; Kratzat, Hanna; Campbell, Annabelle; Buschauer, Robert; Burroughs, A Maxwell; Berninghausen, Otto; Aravind, L; Green, Rachel; Beckmann, Roland; Buskirk, Allen R

Springer Science and Business Media LLC

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