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An optimized bioluminescent substrate for non-invasive imaging in the brain

Nature Chemical Biology, ISSN: 1552-4469, Vol: 19, Issue: 6, Page: 731-739
2023
  • 30
    Citations
  • 0
    Usage
  • 72
    Captures
  • 11
    Mentions
  • 4
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    30
    • Citation Indexes
      29
    • Patent Family Citations
      1
      • Patent Families
        1
  • Captures
    72
  • Mentions
    11
    • News Mentions
      10
      • News
        10
    • Blog Mentions
      1
      • Blog
        1
  • Social Media
    4
    • Shares, Likes & Comments
      4
      • Facebook
        4

Most Recent News

New Study Illuminates Strategies for Bioluminescence Imaging of the Brain

Promega researchers develop NanoLuc-based tool for studying neurological diseases in live models MADISON, Wis.--(BUSINESS WIRE)-- Research published in Nature Chemical Biology demonstrates a new tool

Article Description

Bioluminescence imaging (BLI) allows non-invasive visualization of cells and biochemical events in vivo and thus has become an indispensable technique in biomedical research. However, BLI in the central nervous system remains challenging because luciferases show relatively poor performance in the brain with existing substrates. Here, we report the discovery of a NanoLuc substrate with improved brain performance, cephalofurimazine (CFz). CFz paired with Antares luciferase produces greater than 20-fold more signal from the brain than the standard combination of d-luciferin with firefly luciferase. At standard doses, Antares–CFz matches AkaLuc–AkaLumine/TokeOni in brightness, while occasional higher dosing of CFz can be performed to obtain threefold more signal. CFz should allow the growing number of NanoLuc-based indicators to be applied to the brain with high sensitivity. Using CFz, we achieve video-rate non-invasive imaging of Antares in brains of freely moving mice and demonstrate non-invasive calcium imaging of sensory-evoked activity in genetically defined neurons. [Figure not available: see fulltext.].

Bibliographic Details

Su, Yichi; Walker, Joel R; Hall, Mary P; Klein, Mark A; Wu, Xiang; Encell, Lance P; Casey, Kerriann M; Liu, Lan Xiang; Hong, Guosong; Lin, Michael Z; Kirkland, Thomas A

Springer Science and Business Media LLC

Biochemistry, Genetics and Molecular Biology

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