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Expansion spatial transcriptomics

Nature Methods, ISSN: 1548-7105, Vol: 20, Issue: 8, Page: 1179-1182
2023
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1Shaanxi Provincial Key Laboratory of Infection and Immune Diseases, Shaanxi Provincial People’s Hospital, Xi’an, People’s Republic of China; 2Shaanxi Engineering Research Center of Cell Immunology,

Article Description

Capture array-based spatial transcriptomics methods have been widely used to resolve gene expression in tissues; however, their spatial resolution is limited by the density of the array. Here we present expansion spatial transcriptomics to overcome this limitation by clearing and expanding tissue prior to capturing the entire polyadenylated transcriptome with an enhanced protocol. This approach enables us to achieve higher spatial resolution while retaining high library quality, which we demonstrate using mouse brain samples.

Bibliographic Details

Fan, Yuhang; Andrusivová, Žaneta; Wu, Yunming; Chai, Chew; Larsson, Ludvig; He, Mengxiao; Luo, Liqun; Lundeberg, Joakim; Wang, Bo

Springer Science and Business Media LLC

Biochemistry, Genetics and Molecular Biology

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