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Pre-RC protein MCM7 depletion promotes mitotic exit by inhibiting CDK1 activity

Scientific Reports, ISSN: 2045-2322, Vol: 7, Issue: 1, Page: 2854
2017
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Article Description

MCM7, a subunit of mini-chromosome maintenance proteins (MCM) complex, plays an important role in initiating DNA replication during the G1 phase and extending DNA strands during the S phase. Here, we demonstrated that MCM7 is not only sustained but maintains association with chromatin during M phase. Remarkably, MCM7 siRNA can accelerate mitotic exit. MCM7 depletion leads to CDK1 inactivation and promotes subsequent cohesin/RAD21 cleavage, which eventually leads to sister chromatin segregation. Moreover, MCM7 is co-localized with tubulin in the mitotic cells and MCM7 depletion results in aberrant mitosis. Our results indicate that MCM7 may exert certain functions on spindle formation to prevent cytokinesis during early mitosis by regulating CDK1 activity.

Bibliographic Details

Zheng, Dianpeng; Ye, Sichao; Wang, Xiuyun; Zhang, Yongjun; Yan, Daoyu; Cai, Xiangsheng; Gao, Weihong; Shan, Hongbo; Gao, Yang; Chen, Juanjuan; Hu, Zhiming; Li, Hongwei; Li, Jinlong

Springer Science and Business Media LLC

Multidisciplinary

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