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Thrombopoietin-independent generation of platelet-like particles from megakaryoblastic cells

Scientific Reports, ISSN: 2045-2322, Vol: 13, Issue: 1, Page: 22553
2023
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Article Description

The use of megakaryoblastic leukemia MEG-01 cells can help reveal the mechanisms of thrombopoiesis. However, conventional in vitro activation of platelet release from MEG-01 cells requires thrombopoietin, which is costly. Here, we aim to develop a more straightforward and affordable method. Synchronization of the MEG-01 cells was initially performed using serum-free culture, followed by spontaneous cell differentiation in the presence of serum. Different stages of megakaryoblast differentiation were classified based on cell morphology, DNA content, and cell cycle. The MEG-01 cells released platelet-like particles at a level comparable to that of the thrombopoietin-activated MEG-01 cells. The platelet-like particles were distinguishable from PLP-derived extracellular vesicles and could express P-selectin following ADP activation. Importantly, the platelet-like particles induced fibrin clotting in vitro using platelet-poor plasma. Therefore, this thrombopoietin-independent cell synchronization method is an effective and straightforward method for studying megakaryopoiesis and thrombopoiesis.

Bibliographic Details

Nunthanasup, Nuntiporn; Ketprasit, Nutpakal; Noulsri, Egarit; Palasuwan, Attakorn; Combes, Valery; Kulkeaw, Kasem; Palasuwan, Duangdao

Springer Science and Business Media LLC

Multidisciplinary

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