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Characterisation of the GRAF gene promoter and its methylation in patients with acute myeloid leukaemia and myelodysplastic syndrome

British Journal of Cancer, ISSN: 0007-0920, Vol: 94, Issue: 2, Page: 323-332
2006
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Article Description

We report the isolation of the 5′ flanking region of GRAF (GTPase regulator associated with the focal adhesion kinase), previously described as a putative tumour suppressor gene of acute myelogenous leukaemia and myelodysplastic syndrome, and demonstrate its promoter activity in reporter gene assays. Two putative protein-binding sites are identified of which one was sensitive to CpG methylation. The suppressed GRAF expression could be restored in leukaemia cell lines by treatment with a demethylating agent and an inhibitor of histone deacetylases. In contrast to normal tissues, which tested negative for GRAF promoter methylation, 11 of 29 (38%) bone marrow samples from patients with acute myeloid leukaemia or myelodysplastic syndrome were positive. © 2006 Cancer Research.

Bibliographic Details

S. E. Bojesen; O. Ammerpohl; A. Weinhäusl; O. A. Haas; H. Mettal; R. M. Bohle; A. Borkhardt; U. Fuchs

Springer Science and Business Media LLC

Medicine; Biochemistry, Genetics and Molecular Biology

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