PlumX Metrics
Embed PlumX Metrics

TGF-βl Suppresses Inflammation in Cell Therapy for Intervertebral Disc Degeneration

Scientific Reports, ISSN: 2045-2322, Vol: 5, Issue: 1, Page: 13254
2015
  • 73
    Citations
  • 0
    Usage
  • 46
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

Article Description

Recent studies suggest that cell therapy may be an effective way to repair intervertebral disc degeneration. As a strong immune suppressor, TGF-β1 has been shown to inhibit inflammation respond effectively. The objective of this study was to explore the effects of TGF-β1 during bone marrow mesenchymal stem cells-based therapy for disc degeneration. In vitro assays demonstrated that co-culturing of nucleus pulposus cells with bone marrow mesenchymal stem cells resulted in significantly higher levels of TGF-βl secretion. This increase inhibited l κ B phosphorylation and NF-κB activation, detected by western blot analysis. Meanwhile, in a rabbit model, MRI analysis revealed significant recovery of signal intensity in the degenerative discs of rabbits receiving cells transplantation, than receiving cells treated with a TGF-β1 inhibitor or saline. These findings indicated that enhanced TGF-β1 production recovered the degeneration of intervertebral disc. And also immunohistochemical staining detected enhanced collagen II expression in the rabbits treated with cell transplantation. However, the NF-κ B positive cells were significantly less than other two control groups. Thus, cell therapy promoted TGF-β1 expression in nucleus pulposus, leading to anti-inflammatory effects via the inhibition of NF-κ B, and the amelioration of disc degradation due to increased expression of collagen II and aggrecan in degenerative intervertebral disc.

Bibliographic Details

Yang, Huilin; Cao, Cheng; Wu, Chunshen; Yuan, Chenxi; Gu, Qiaoli; Shi, Qing; Zou, Jun

Springer Science and Business Media LLC

Multidisciplinary

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know