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DETC-based bacterial cellulose bio-curatives for topical treatment of cutaneous leishmaniasis

Scientific Reports, ISSN: 2045-2322, Vol: 6, Issue: 1, Page: 38330
2016
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Article Description

The treatment of leishmaniasis still relies on drugs with potentially serious adverse effects. Herein, we tested a topical formulation of bacterial cellulose (BC) membranes containing Diethyldithiocarbamate (DETC), a superoxide dismutase 1 inhibitor. Leishmania-infected macrophages exposed to BC-DETC resulted in parasite killing, without pronounced toxic effects to host cells. This outcome was associated with lower SOD1 activity and higher production of superoxide and cytokine mediators. Topical application of BC-DETC significantly decreased lesion size, parasite load and the inflammatory response at the infection site, as well as the production of both IFN-γ and TNF. Combination of topical BC-DETC plus intraperitoneal Sb v also significantly reduced disease development and parasite load. The leishmanicidal effect of BC-DETC was extended to human macrophages infected with L. braziliensis, highlighting the feasibility of BC-DETC as a topical formulation for chemotherapy of cutaneous leishmaniasis caused by L. braziliensis.

Bibliographic Details

Celes, Fabiana S; Trovatti, Eliane; Khouri, Ricardo; Van Weyenbergh, Johan; Ribeiro, Sidney J L; Borges, Valeria M; Barud, Hernane S; de Oliveira, Camila I

Springer Science and Business Media LLC

Multidisciplinary

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