Photoactivated adenylyl cyclase (PAC) genes in the flagellate Euglena gracilis mutant strains
Photochemical and Photobiological Sciences, ISSN: 1474-9092, Vol: 4, Issue: 9, Page: 732-739
2005
- 12Citations
- 13Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations12
- Citation Indexes12
- 12
- CrossRef10
- Captures13
- Readers13
- 13
Article Description
The unicellular, green flagellate wild-type Euglena gracilis(strain Z) and its colorless phototaxis-mutant strains as well as the non-photosynthetic close relative, Astasia longa, possess several genes of the photoactivated adenylyl cyclase (PAC) family. The corresponding gene products were found to be responsible for step-up (but not step-down) photophobic responses as well as both positive and negative phototaxis. The proteins consist of two PACα(M 105 kDa) and two PACβ(90 kDa) subunits. While the proteins were first believed all to be located in the paraxonemal body (PAB), confocal microscopy revealed that Astasia longa as well as some of the mutant strains do not contain a PAB. Immunofluorescence using PAC antibodies showed that the PAC proteins are also located along the total length of the flagellum at least in some of the strains. In order to determine if the genes responsible for the PAC proteins in the PAB and flagella are identical, sequences of all PAC proteins were analyzed in the Euglena and Astasia strains studied for PAC protein location. Full sequence analysis using PCR and 3′ and 5′ RACE indicated a substantial divergence between strains with a homology between strains of between 45 and 100%. Sequence alignment and sequence tree construction for the main functional groups (BLUF domain, which binds FAD, and adenylyl cyclase) showed that the pacα and the pacβ gene products form clusters each with some of the mutants being closely related while others show a substantial degree of genetic diversity. The conclusion of these results is that there is a family of very dissimilar PAC proteins located in the PAB and the flagellum where they serve different functions in phototaxis and step-up photophobic reactions. © 2005 The Royal Society of Chemistry and Owner Societies.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=25444437474&origin=inward; http://dx.doi.org/10.1039/b502002f; http://www.ncbi.nlm.nih.gov/pubmed/16121285; https://link.springer.com/10.1039/b502002f; https://dx.doi.org/10.1039/b502002f; https://link.springer.com/article/10.1039/b502002f
Springer Science and Business Media LLC
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know