Sequence-selective osmium oxidation of DNA: Efficient distinction between 5-methylcytosine and cytosine
Organic and Biomolecular Chemistry, ISSN: 1477-0520, Vol: 4, Issue: 9, Page: 1638-1640
2006
- 53Citations
- 19Captures
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Metrics Details
- Citations53
- Citation Indexes52
- 52
- CrossRef47
- Patent Family Citations1
- Patent Families1
- Captures19
- Readers19
- 19
Article Description
A common modified pyrimidine base, 5-Methylcytosine (M) was distinguished from cytosine using the large difference of their osmium oxidation rates, and this reaction was applied to detection of the cytosine methylation status at a specific site of a long sequence. This was done using the formation of a bulge structure by hybridization with a guide DNA. 5-Methylcytosine is a common modified pyrimidine base, which frequently appears in genomic DNA, particularly in CpG sequences, and plays a key role in epigenetic events. M was oxidized efficiently by exposure to a reaction mixture containing an osmium complex, making possible a clear distinction from very weak oxidation of C. M-selective oxidation is applicable to detection of the cytosine methylation status at a specific site of a long sequence using the formation of a bulge structure by hybridization with a guide DNA.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33744821360&origin=inward; http://dx.doi.org/10.1039/b600401f; http://www.ncbi.nlm.nih.gov/pubmed/16633552; https://xlink.rsc.org/?DOI=b600401f; https://dx.doi.org/10.1039/b600401f; https://pubs.rsc.org/en/content/articlelanding/2006/ob/b600401f
Royal Society of Chemistry (RSC)
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