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Motile and non-motile sperm diagnostic manipulation using optoelectronic tweezers

Lab on a Chip, ISSN: 1473-0189, Vol: 10, Issue: 23, Page: 3213-3217
2010
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Article Description

Optoelectronic tweezers was used to manipulate human spermatozoa to determine whether their response to OET predicts sperm viability among non-motile sperm. We review the electro-physical basis for how live and dead human spermatozoa respond to OET. The maximal velocity that non-motile spermatozoa could be induced to move by attraction or repulsion to a moving OET field was measured. Viable sperm are attracted to OET fields and can be induced to move at an average maximal velocity of 8.8 ± 4.2 m s, while non-viable sperm are repelled to OET, and are induced to move at an average maximal velocity of -0.8 ± 1.0 m s. Manipulation of the sperm using OET does not appear to result in increased DNA fragmentation, making this a potential method by which to identify viable non-motile sperm for assisted reproductive technologies. © 2010 The Royal Society of Chemistry.

Bibliographic Details

Ohta, Aaron T; Garcia, Maurice; Valley, Justin K; Banie, Lia; Hsu, Hsan-Yin; Jamshidi, Arash; Neale, Steven L; Lue, Tom; Wu, Ming C

Royal Society of Chemistry (RSC)

Chemical Engineering; Biochemistry, Genetics and Molecular Biology; Chemistry; Engineering

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