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Bioethanol production from marine biomass alginate by metabolically engineered bacteria

Energy and Environmental Science, ISSN: 1754-5692, Vol: 4, Issue: 7, Page: 2575-2581
2011
  • 155
    Citations
  • 0
    Usage
  • 141
    Captures
  • 0
    Mentions
  • 33
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    155
    • Citation Indexes
      155
  • Captures
    141
  • Social Media
    33
    • Shares, Likes & Comments
      33
      • Facebook
        33

Article Description

Bioethanol production from algae is a promising approach that resolves problems associated with biofuel production from land biomass, such as bioethanol-food conflicts and the indirect land use change. However, it presents several technical difficulties because existing ethanologenic microbes can neither degrade alginate, a major component of brown algae, nor assimilate alginate degradation products. We developed an integrated bacterial system for converting alginate to ethanol using a metabolically modified, alginate-assimilating, pit-forming bacterium, Sphingomonas sp. A1 (strain A1). Overexpression of Zymomonas mobilis pdc and adhB was achieved using a strong constitutive expression promoter newly identified in strain A1 and by inserting multiple gene copies using the methylation sensitivity of XbaI. Metabolome analysis revealed by-product accumulation, and its synthesis pathway was blocked by gene disruption. The ethanologenic recombinant strain A1 accumulated 13.0 g L ethanol in 3 d using alginate as the sole carbon source. © 2011 The Royal Society of Chemistry.

Bibliographic Details

Hiroyuki Takeda; Fuminori Yoneyama; Shigeyuki Kawai; Wataru Hashimoto; Kousaku Murata

Royal Society of Chemistry (RSC)

Environmental Science; Energy

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