Molecular rheometry: Direct determination of viscosity in L and L lipid phases via fluorescence lifetime imaging
Physical Chemistry Chemical Physics, ISSN: 1463-9076, Vol: 15, Issue: 36, Page: 14986-14993
2013
- 157Citations
- 181Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations157
- Citation Indexes157
- 157
- CrossRef147
- Captures181
- Readers181
- 181
Article Description
Understanding of cellular regulatory pathways that involve lipid membranes requires the detailed knowledge of their physical state and structure. However, mapping the viscosity and diffusion in the membranes of complex composition is currently a non-trivial technical challenge. We report fluorescence lifetime spectroscopy and imaging (FLIM) of a meso-substituted BODIPY molecular rotor localised in the leaflet of model membranes of various lipid compositions. We prepare large and giant unilamellar vesicles (LUVs and GUVs) containing phosphatidylcholine (PC) lipids and demonstrate that recording the fluorescence lifetime of the rotor allows us to directly detect the viscosity of the membrane leaflet and to monitor the influence of cholesterol on membrane viscosity in binary and ternary lipid mixtures. In phase-separated 1,2-dioleoyl-sn-glycero-3- phosphocholine-cholesterol-sphingomyelin GUVs we visualise individual liquid ordered (L) and liquid disordered (L) domains using FLIM and assign specific microscopic viscosities to each domain. Our study showcases the power of FLIM with molecular rotors to image microviscosity of heterogeneous microenvironments in complex biological systems, including membrane-localised lipid rafts. © the Owner Societies 2013.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84882963902&origin=inward; http://dx.doi.org/10.1039/c3cp51953h; http://www.ncbi.nlm.nih.gov/pubmed/23912893; https://xlink.rsc.org/?DOI=c3cp51953h; https://dx.doi.org/10.1039/c3cp51953h; https://pubs.rsc.org/en/content/articlelanding/2013/cp/c3cp51953h
Royal Society of Chemistry (RSC)
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