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Investigation of end processing and degradation of premature tRNAs and their application to stabilization of in vitro transcripts in wheat germ extract

Organic and Biomolecular Chemistry, ISSN: 1477-0520, Vol: 13, Issue: 4, Page: 1008-1012
2015
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Article Description

We investigated the end processing and degradation of premature tRNAs in wheat germ extract (WGE), which led to the discovery of end protectors useful for stabilizing an in vitro transcript against various ribonucleases and thereby enhancing its apparent activity in WGE.

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