A comparative study of three different nucleic acid amplification techniques combined with microchip electrophoresis for HPV16 E6/E7 mRNA detection
Analyst, ISSN: 1364-5528, Vol: 140, Issue: 19, Page: 6736-6741
2015
- 21Citations
- 17Captures
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Metrics Details
- Citations21
- Citation Indexes21
- 21
- CrossRef20
- Captures17
- Readers17
- 17
Article Description
Research towards nucleic acid amplification technologies for detection of human papillomavirus (HPV) 16 E6/E7 mRNA was carried out in combination with microchip electrophoresis (MCE). The approaches of nucleic acid sequence based amplification (NASBA), one-step RT-PCR and two-step RT-PCR were successfully developed. NASBA was a simple enzymatic reaction, which directly amplified HPV16 mRNA by isothermal amplification, leaving out the complex and tedious operation. One-step RT-PCR simplified the amplification step, while two-step RT-PCR was more sensitive and less vulnerable to the interference. Furthermore, instead of gel electrophoresis, microchip electrophoresis (MCE) for RNA assay was employed to realize high-throughput and rapid analysis. Finally, the results show that PCR-based or NASBA-based mRNA tests are valuable for HPV mRNA assay, which can be potentially applied for clinical diagnosis and prognosis of cervical and other anogenital carcinoma.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84941703018&origin=inward; http://dx.doi.org/10.1039/c5an00944h; http://www.ncbi.nlm.nih.gov/pubmed/26332096; https://xlink.rsc.org/?DOI=C5AN00944H; http://xlink.rsc.org/?DOI=C5AN00944H; http://pubs.rsc.org/en/content/articlepdf/2015/AN/C5AN00944H; https://dx.doi.org/10.1039/c5an00944h; https://pubs.rsc.org/en/content/articlelanding/2015/an/c5an00944h
Royal Society of Chemistry (RSC)
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