Ratiometric fluorescence sensor arrays based on quantum dots for detection of proteins
Analyst, ISSN: 1364-5528, Vol: 141, Issue: 6, Page: 2046-2052
2016
- 35Citations
- 25Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations35
- Citation Indexes35
- 35
- CrossRef33
- Captures25
- Readers25
- 25
Article Description
Optical cross-reactive sensor arrays have recently been demonstrated as a powerful tool for high-throughput protein analysis. Nevertheless, applying this technology to protein detection is complicated by many external factors, such as the interfering substances, the background noise, and sample environmental changes in the biological matrix. Herein we demonstrate that a ratiometric fluorescence sensor array based on quantum dots can be employed to circumvent these limitations. Several intrinsic dual-emitting Mn-doped quantum dots capped with different organic functional groups were designed as sensing elements. Distinct and reproducible response patterns against the ratiometric sensor array were obtained from ten proteins in a buffer of different pH (pH 5.7, 7.4, and 8.3) and spiked into human urine. Linear discrimination analysis of the response patterns showed successful differentiation of the analytes at concentrations as low as 50 nM with high identification accuracy. Furthermore, this sensor system also enables the detection of these eight proteins (at 500 nM) in human urine without any treatment. The ratiometric fluorescence change from quantum dots for analysis of proteins can eliminate effectively the signal interference from the pH value change and the fluorescent background in human urine. The present study will open a new avenue to improve the discrimination ability of sensor arrays.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84960379115&origin=inward; http://dx.doi.org/10.1039/c5an02545a; http://www.ncbi.nlm.nih.gov/pubmed/26858999; http://xlink.rsc.org/?DOI=C5AN02545A; http://pubs.rsc.org/en/content/articlepdf/2016/AN/C5AN02545A; https://xlink.rsc.org/?DOI=C5AN02545A; https://dx.doi.org/10.1039/c5an02545a; https://pubs.rsc.org/en/content/articlelanding/2016/an/c5an02545a
Royal Society of Chemistry (RSC)
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