An on-demand gas segmented flow generator with high spatiotemporal resolution for: In vivo analysis of neuronal response in C. elegans
Lab on a Chip, ISSN: 1473-0189, Vol: 16, Issue: 20, Page: 4020-4027
2016
- 10Citations
- 17Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations10
- Citation Indexes10
- 10
- CrossRef9
- Captures17
- Readers17
- 17
Article Description
Studies of chemo-sensing in C. elegans to fluctuating gaseous cues are limited due to the lack of a method of precise gas control. In this paper, we describe a microfluidic-based on-demand gas segmented flow generator for performing fluctuating gaseous stimulations to worms. This highly versatile and programmable micro-device integrated with pneumatic valves for flexible and stable gas flow control and worm immobilization enabled us to examine the temporal features of neuronal response to multiple gas pulses with sub-second precision. As a result, we demonstrated the capability of the micro-device to generate repetitive gaseous chemical pulses with varying durations. By characterizing intracellular calcium signals, we showed that URX sensory neurons were sensitive to O pulses with duration of more than 0.5 s. Furthermore, URX neuronal adaptation and recovery in response to gaseous chemical pulses were investigated by varying the durations and intervals. The developed microfluidic system is shown to be a useful tool for studying the dynamics of in vivo gas-evoked neuronal responses and revealing the temporal properties of environmental stimulations.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84990841894&origin=inward; http://dx.doi.org/10.1039/c6lc00948d; http://www.ncbi.nlm.nih.gov/pubmed/27714011; https://xlink.rsc.org/?DOI=C6LC00948D; http://xlink.rsc.org/?DOI=C6LC00948D; http://pubs.rsc.org/en/content/articlepdf/2016/LC/C6LC00948D; https://dx.doi.org/10.1039/c6lc00948d; https://pubs.rsc.org/en/content/articlelanding/2016/lc/c6lc00948d
Royal Society of Chemistry (RSC)
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