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Modulating the GSH/Trx selectivity of a fluorogenic disulfide-based thiol sensor to reveal diminished GSH levels under ER stress

Chemical Communications, ISSN: 1364-548X, Vol: 54, Issue: 64, Page: 8897-8900
2018
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Article Description

We synthesized a fluorogenic disulfide-based naphthalimide thiol probe (ER-Naph) with a hydrophilic endoplasmic reticulum (ER)-guiding glibenclamide unit. Its ER targeting ability and high selectivity to GSH over thioredoxin, a potent competitor, were clearly demonstrated, both in solution and in vitro. Finally, a confocal microscopic investigation revealed that GSH levels in the ER were dramatically decreased under thapsigargin, brefeldin A, and tunicamycin-induced ER stress models.

Bibliographic Details

Wi, Youngjin; Le, Hoa Thi; Verwilst, Peter; Sunwoo, Kyoung; Kim, Seo Jin; Song, Jung Eun; Yoon, Hey Young; Han, Geon; Kim, Jong Seung; Kang, Chulhun; Kim, Tae Woo

Royal Society of Chemistry (RSC)

Chemical Engineering; Materials Science; Chemistry

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