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The oxygen reactivity of an artificial hydrogenase designed in a reengineered copper storage protein

Dalton Transactions, ISSN: 1477-9234, Vol: 49, Issue: 6, Page: 1928-1934
2020
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The O reactivity of an artificial biomolecular hydrogenase, the nickel binding protein (NBP) is investigated. Kinetic analyses revealed a complete 4e reduction of O to HO under catalytic conditions with associated k for ET in the order of 10 cm s. Protein destabilization and S oxygenation are contributing factors to the deactivation of NBP under oxic conditions. Computational studies provided insight into the S oxygenation and the reaction intermediates of a proposed mechanistic pathway for O activation by NBP.

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