Differential protein expression of a streptomycin-resistant: Streptomyces albulus mutant in high yield production of ϵ-poly-l-lysine: A proteomics study
RSC Advances, ISSN: 2046-2069, Vol: 9, Issue: 42, Page: 24092-24104
2019
- 11Citations
- 9Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations11
- Citation Indexes11
- CrossRef11
- 11
- Captures9
- Readers9
Article Description
ϵ-Poly-l-lysine (ϵ-PL), produced by Streptomyces albulus, is an excellent antimicrobial agent which has been extensively used in the field of food and medicine. In our previous study, we have improved ϵ-PL production by S. albulus M-Z18 through iterative introduction of streptomycin resistance. To decipher the overproduction mechanism of high-yielding mutant S. albulus SS-62, we conducted a comparative proteomics analysis between S. albulus SS-62 and its parent strain S. albulus M-Z18. Approximately 11.5% of the predicted S. albulus proteome was detected and 401 known or putative regulatory proteins showed statistically differential expression levels. Expression levels of proteins involved in ϵ-PL precursor metabolism and energy metabolism, and proteins in the pathways related to transcriptional regulation and translation were up-regulated. It was indicated that mutant SS-62 could not only strengthen the ϵ-PL precursor metabolism and energy metabolism but also tune the pathways related to transcriptional regulation and translation, suggesting a better intracellular metabolic environment for the synthesis of ϵ-PL in mutant SS-62. To confirm these bioinformatics analyses, qRT-PCR was employed to investigate the transcriptional levels of pls, frr and hrdD and their transcription levels were found to have increased more than 4-fold. Further, overexpression of pls and frr resulted in an increase in ϵ-PL titer and the yield of ϵ-PL per unit cell. This report not only represents the first comprehensive study on comparative proteomics in S. albulus, but it would also guide strain engineering to further improve ϵ-PL production.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85070470953&origin=inward; http://dx.doi.org/10.1039/c9ra03156a; http://www.ncbi.nlm.nih.gov/pubmed/35527895; https://xlink.rsc.org/?DOI=C9RA03156A; https://dx.doi.org/10.1039/c9ra03156a; https://pubs.rsc.org/en/content/articlelanding/2019/ra/c9ra03156a
Royal Society of Chemistry (RSC)
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