A natural cyanobacterial protein C-phycoerythrin as an Hg selective fluorescent probe in aqueous systems
New Journal of Chemistry, ISSN: 1369-9261, Vol: 44, Issue: 16, Page: 6601-6609
2020
- 11Citations
- 6Captures
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Article Description
A natural, yellow fluorescent cyanobacterial protein, C-phycoerythrin (CPE), was purified from a marine cyanobacterium Lyngbya sp. to a purity ratio of 5.17 using ammonium sulphate precipitation and column chromatographic techniques. After characterization by UV-visible and fluorescence spectroscopy, it was found to selectively detect micromolar quantities of Hg among 12 biologically significant metal cations through the fluorescence 'turn off' phenomenon. Sequential titration of CPE with Hg revealed a minor hypsochromic and a major hypochromic shift in its absorbance maximum and significant fluorescence quenching above 2 μM Hg. The response of CPE towards Hg was instantaneous and unaffected by high concentrations of other cations except Cu. CPE was able to selectively detect and quantify Hg in pond, tap and effluent water samples with recovery % of ∼94.8-107.5%. FT-IR analysis suggested Hg interactions with -NH regions in the protein. Thus, CPE could be further explored as a natural agent for the selective detection of biologically and environmentally hazardous Hg through the fluorescence 'turn off' route.
Bibliographic Details
Royal Society of Chemistry (RSC)
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