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Construction of polysiloxane-based fluorescent probe for visualizing pH down-regulation

Journal of Materials Chemistry C, ISSN: 2050-7526, Vol: 9, Issue: 7, Page: 2392-2397
2021
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Article Description

Changes in the intracellular pH values constitute a significant physiological and pathological process and play vital roles in autophagy, self-repairing, and programmed cell apoptosis. A unique fluorescent probe (PN-1) based on polysiloxanes designed using the intramolecular charge transfer (ICT) mechanism has been developed for the detection of the pH down-regulation process for the first time.PN-1has been successfully applied to HeLa cells and HepG2 cells for visualizing the pH value changes in a ratiometric manner. The result presented that, in the normal intracellular autophagy process,PN-1displayed strong blue fluorescence while starvation induced apparent red fluorescence ofPN-1. In addition, the inhibitory effect of chloroquine (CQ) to pH value changes during the cell starvation treatment process has been analyzed. The work indicated that a polysiloxane-based fluorescent probe could be applied to detect pH value changes during autophagy, and paved a new avenue for the further design of fluorescent probes for monitoring pH changes.

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