PlumX Metrics
Embed PlumX Metrics

Solvent effects of N,N-dimethylformamide and methanol on mass spectrometry imaging by tapping-mode scanning probe electrospray ionization

Analyst, ISSN: 1364-5528, Vol: 148, Issue: 6, Page: 1275-1284
2023
  • 6
    Citations
  • 0
    Usage
  • 5
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

Article Description

Mass spectrometry imaging (MSI) is an effective technique for visualizing the distribution of lipids in tissues. The direct extraction-ionization methods using minute volumes of solvent for local components have the advantage of rapid measurement without any sample pretreatment. For effective MSI of tissues, it is necessary to understand the effect of solvent physicochemical properties on ion images. In this study, we report solvent effects on the lipid imaging of mouse brain tissue by tapping-mode scanning probe electrospray ionization (t-SPESI) which is capable of extraction-ionization using sub-pL solvents. To precisely measure lipid ions, we developed a measurement system incorporating a quadrupole-time-of-flight mass spectrometer. The differences in signal intensity and spatial resolution of lipid ion images were investigated using N,N-dimethylformamide (non-protic polar solvent), methanol (protic polar solvent) and their mixture. The mixed solvent was suitable for the protonation of lipids, and it provided high spatial resolution MSI. Results indicate that the mixed solvent improves the extractant transfer efficiency and minimizes charged droplets from an electrospray. The solvent selectivity study revealed the importance of solvent selection based on physicochemical properties for the advancement of MSI by t-SPESI.

Bibliographic Details

Otsuka, Yoichi; Ote, Nijiho; Sun, Mengze; Shimma, Shuichi; Urakawa, Osamu; Yamaguchi, Shinichi; Kudo, Tomoya; Toyoda, Michisato

Royal Society of Chemistry (RSC)

Chemistry; Biochemistry, Genetics and Molecular Biology; Environmental Science

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know