A LOV-domain-mediated blue-light-activated adenylate (adenylyl) cyclase from the cyanobacterium Microcoleus chthonoplastes PCC 7420
Biochemical Journal, ISSN: 0264-6021, Vol: 455, Issue: 3, Page: 359-365
2013
- 59Citations
- 58Captures
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Metrics Details
- Citations59
- Citation Indexes59
- 59
- CrossRef48
- Captures58
- Readers58
- 58
Article Description
Genome screening of the cyanobacterium Microcoleus chthonoplastes PCC 7420 identified a gene encoding a protein (483 amino acids, 54.2 kDa in size) characteristic of a BL (blue light)-regulated adenylate (adenylyl) cyclase function. The photoreceptive part showed signatures of a LOV (light, oxygen, voltage) domain. The gene product, mPAC (Microcoleus photoactivated adenylate cyclase), exhibited the LOV-specific three-peaked absorption band (λ =450 nm) and underwent conversion into the photoadduct form (λ =390 nm) upon BLirradiation. The lifetime for thermal recovery into the parent state was determined as 16 s at 20°C (25 s at 11°C). The adenylate cyclase function showed a constitutive activity (in the dark) that was in-vitro-amplified by a factor of 30 under BL-irradiation. Turnover of the purified protein at saturating light and pH 8 is estimated to 1 cAMP/mPAC per s at 25°C (2 cAMP/mPAC per s at 35°C). The lifetime of light-activated cAMP production after a BL flash was ~14 s at 20°C. The temperature optimum was determined to 35°C and the pH optimum to 8.0. The value for half-maximal activating light intensity is 6 W/m (at 35°C). A comparison of mPAC and the BLUF (BL using FAD) protein bPAC (Beggiatoa PAC), as purified proteins and expressed in Xenopus laevis oocytes, yielded higher constitutive activity for mPAC in the dark, but also when illuminated with BL. © 2013 Biochemical Society.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84885579044&origin=inward; http://dx.doi.org/10.1042/bj20130637; http://www.ncbi.nlm.nih.gov/pubmed/24112109; https://portlandpress.com/biochemj/article/455/3/359/46216/A-LOV-domain-mediated-blue-light-activated; http://biochemj.org/lookup/doi/10.1042/BJ20130637; https://dx.doi.org/10.1042/bj20130637; https://portlandpress.com/biochemj/article-abstract/455/3/359/46216/A-LOV-domain-mediated-blue-light-activated?redirectedFrom=fulltext
Portland Press Ltd.
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