Polyclonal-antibody-mediated insolubilization and stabilization of papain
Biotechnology and Applied Biochemistry, ISSN: 0885-4513, Vol: 32, Issue: 2, Page: 89-94
2000
- 8Citations
- 6Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations8
- Citation Indexes8
- CrossRef7
- Captures6
- Readers6
Article Description
Antisera raised in rabbits against native iodoacetamide- and iodoacetic acid-modified papain contained precipitating and non-inhibitory antibodies. Papain could be insolubilized as enzyme-antibody adducts with the γ-globulin fraction derived from the antiserum. Two insolubilized papain preparations, designated A and B, with low and high antibody/enzyme ratios, respectively, exhibited high enzyme activity and markedly enhanced thermal and denaturant stabilities. However, papain antibody adduct B was superior in activity and stability over preparation A. The usefulness of immobilized papain in biopharmaceutical and other industries is also discussed.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0033778711&origin=inward; http://dx.doi.org/10.1042/ba19990091; http://www.ncbi.nlm.nih.gov/pubmed/11001869; https://iubmb.onlinelibrary.wiley.com/doi/10.1042/BA19990091; http://doi.wiley.com/10.1042/BA19990091; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1042%2FBA19990091; https://dx.doi.org/10.1042/ba19990091; https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1042/BA19990091
Wiley
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