Calcium modulation of exocytosis-linked plasma membrane potential oscillations in INS-1 832/13 cells
Biochemical Journal, ISSN: 1470-8728, Vol: 471, Issue: 1, Page: 111-122
2015
- 10Citations
- 6Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations10
- Citation Indexes10
- 10
- CrossRef8
- Captures6
- Readers6
Article Description
In the presence of high glucose or pyruvate, INS-1 832/13 insulinoma cells undergo stochastic oscillations in plasma membrane potential (Δψ) leading to associated fluctuations in cytosolic free Ca concentration ([Ca]). Oscillations are not driven by upstream metabolic fluctuations, but rather by autonomous ionic mechanisms, the details of which are unclear. We have investigated the nature of the oscillator, with simultaneous fluorescence monitoring of Δψ, [Ca] and exocytosis at single-cell resolution, combined with analysis of the occurrence, frequency and amplitude of Δψ oscillations. Oscillations were closely coupled to exocytosis, indicated by coincident synaptopHluorin fluorescence enhancement. L-type Ca channel inhibitors enhanced Δψ and [Ca] oscillation frequency in the presence of pyruvate, but abolished the sustained [Ca] response following KCl depolarization. The L-type Ca channel inhibitor isradipine did not inhibit oscillation-linked exocytosis. The T-type Ca channel inhibitor NNC 55-0396 inhibited Δψ and [Ca] oscillations, implying that T-type Ca channels trigger oscillations and consequent exocytosis. Since distinct ion channels operate in oscillating and non-oscillating cells, quantitative analysis of Δψ and [Ca] oscillations in a β-cell population may help to improve our understanding of the link between metabolism and insulin secretion.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84942354220&origin=inward; http://dx.doi.org/10.1042/bj20150616; http://www.ncbi.nlm.nih.gov/pubmed/26243883; http://biochemj.org/cgi/doi/10.1042/BJ20150616; https://portlandpress.com/biochemj/article/471/1/111/48630/Calcium-modulation-of-exocytosis-linked-plasma; https://dx.doi.org/10.1042/bj20150616; https://portlandpress.com/biochemj/article-abstract/471/1/111/48630/Calcium-modulation-of-exocytosis-linked-plasma?redirectedFrom=fulltext
Portland Press Ltd.
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