PlumX Metrics
Embed PlumX Metrics

Periodically fluctuating protein kinases phosphorylate CLOCK, the putative target in the suprachiasmatic nucleus

Journal of Neurochemistry, ISSN: 0022-3042, Vol: 72, Issue: 5, Page: 2191-2197
1999
  • 13
    Citations
  • 0
    Usage
  • 16
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

Article Description

We studied nuclear protein phosphorylation in the rat suprachiasmatic nucleus (SCN) and found that a nuclear fraction of the SCN contained histone H1 kinase activity that periodically fluctuated with a diurnal rhythm, reaching a maximum at the midpoint of the light phase and a minimum at the midpoint of the dark phase. A p13(suc1)-bound fraction from the SCN nuclear fraction also exhibited diurnally fluctuating histone H1 kinase activity. Using in situ kinase assay, three histone H1 kinases, p45(PFK), p100(PFK), and p200(PFK) (termed periodically fluctuating protein kinases, or PFKs) were found in the p13(suc1)-bound fractions. p45(PFK) exhibited the highest level of light/dark cycle phosphorylation activity fluctuation. p45(PFK) highly phosphorylated the Ser-Pro-rich region of CLOCK, the putative physiological target. These results suggest that PFKs, especially p45(PFK) are involved in circadian clock-related signal transduction and gene expression, through the phosphorylation of target proteins such as CLOCK.

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know