PlumX Metrics
Embed PlumX Metrics

Differentiation of Murine Melanocyte Precursors Induced by 1,25-Dihydroxyvitamin D 3 Is Associated with the Stimulation of Endothelin B Receptor Expression

Journal of Investigative Dermatology, ISSN: 0022-202X, Vol: 119, Issue: 3, Page: 583-589
2002
  • 56
    Citations
  • 0
    Usage
  • 32
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

Article Description

The effects of 1,25-dihydroxyvitamin D 3 on the differentiation of immature melanocyte precursors were studied. The NCC-/melb4 cell line is an immature melanocyte cell line established from mouse neural crest cells. 1,25-dihydroxyvitamin D 3 inhibited the growth of NCC-/melb4 cells at concentrations higher than 10 -8 M. That growth inhibition was accompanied by the induction of tyrosinase and a change in L-3,4-dihydroxyphenylalanine reactivity from negative to positive. Electron microscopy demonstrated that melanosomes were in more advanced stages after 1,25-dihydroxyvitamin D 3 treatment. In primary cultures of murine neural crest cells, L-3,4-dihydroxyphenylalanine-positive cells were increased after 1,25-dihydroxyvitamin D 3 treatment. These findings indicate that 1,25-dihydroxyvitamin D 3 stimulates the differentiation of immature melanocyte precursors. Moreover, immunostaining and reverse transcription–polymerase chain reaction analysis revealed that endothelin B receptor expression was induced in NCC-/melb4 cells following treatment with 1,25-dihydroxyvitamin D 3. The induction of endothelin B receptor by 1,25-dihydroxyvitamin D 3 was also demonstrated in neural crest cell primary cultures, but not in mature melanocytes. The expression of microphthalmia-associated transcription factor was induced in NCC-/melb4 cells treated with 1,25-dihydroxyvitamin D 3 and endothelin 3, but not by 1,25-dihydroxyvitamin D 3 alone, suggesting that endothelin 3 may stimulate the expression of the microphthalmia-associated transcription factor gene after binding to the endothelin B receptor induced by 1,25-dihydroxyvitamin D 3. These findings suggest a regulatory role for vitamin D 3 in melanocyte development and melanogenesis, and may also explain the working mechanism of vitamin D 3 in the treatment of vitiligo.

Bibliographic Details

Watabe, Hidenori; Soma, Yoshinao; Kawa, Yoko; Ito, Masaru; Ooka, Shiho; Ohsumi, Kayoko; Baba, Takako; Kawakami, Tamihiro; Hosaka, Eri; Kimura, Satoko; Mizoguchi, Masako

Elsevier BV

Biochemistry, Genetics and Molecular Biology; Medicine

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know