PlumX Metrics
Embed PlumX Metrics

Substance P Enhances the Production of Interferon-induced Protein of 10 kDa by Human Keratinocytes in Synergy with Interferon-γ

Journal of Investigative Dermatology, ISSN: 0022-202X, Vol: 119, Issue: 6, Page: 1290-1297
2002
  • 26
    Citations
  • 0
    Usage
  • 11
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

Article Description

A neuropeptide substance P is related to skin inflammation. Interferon-induced protein of 10 kDa (IP-10) chemoattracts T helper 1 cells, and interferon-induced protein of 10 kDa production by keratinocytes is enhanced in inflammatory skin diseases such as psoriasis. We examined the in vitro effects of substance P on interferon-induced protein of 10 kDa production by human keratinocytes. Though substance P alone did not induce interferon-induced protein of 10 kDa production, it enhanced interferon-induced protein of 10 kDa secretion, mRNA expression, and promoter activity induced by suboptimal concentrations of interferon-γ. Interferon-stimulated response element and two nuclear factor-κB sites on interferon-induced protein of 10 kDa promoter were responsible for the enhancement by substance P. Substance P alone enhanced transcriptional activity and transcription factor binding through the two nuclear factor-κB sites, whereas it did not alter interferon-γ-induced transcriptional activity and transcription factor binding through interferon-stimulated response element. The effects of substance P on interferon-induced protein of 10 kDa production and nuclear factor-κB activation were inhibited by neurokinin-1 receptor antagonist, phospholipase C inhibitor, intracellular Ca 2+ chelator, and anti-oxidant. These results suggest that substance P may induce nuclear factor-κB activation and interferon-induced protein of 10 kDa production in synergy with interferon-γ via neurokinin-1 receptor on keratinocytes. These effects of substance P may be mediated via phospholipase C activation, intra-cellular Ca 2+ signal, and reactive oxygen intermediates.

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know