An enzyme-linked immunosorbent assay on a centrifugal platform using magnetic beads
Biomicrofluidics, ISSN: 1932-1058, Vol: 8, Issue: 5, Page: 052110
2014
- 10Citations
- 21Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations10
- Citation Indexes10
- 10
- CrossRef8
- Captures21
- Readers21
- 21
Article Description
An automated, disk-based, enzyme-linked immunosorbent assay (ELISA) system is presented in this work. Magnetic beads were used as the antibody carriers to improve the assay sensitivity and shorten the reaction time. The magnetic module integrated on the system is capable of controlling the magnetic beads to either move in the incubation stage or immobilize at a specific location during washing stage. This controlling mechanism utilizes a passive controlling approach so that it can be performed through disk spinning without the need of active control from external devices. The movement of the magnetic beads was investigated and the optimal rotational speed was found to be related to the ratio of the processing time to the cycle time of the magnetic beads. Comparing to ELISA conducted on microtiter plates, similar test results could be achieved by the disk-based ELISA but the entire protocol can be finished automatically within 45 min with much less reagent consumption.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84907412572&origin=inward; http://dx.doi.org/10.1063/1.4896297; http://www.ncbi.nlm.nih.gov/pubmed/25538803; https://pubs.aip.org/bmf/article/8/5/052110/386113/An-enzyme-linked-immunosorbent-assay-on-a; http://scitation.aip.org/content/aip/journal/bmf/8/5/10.1063/1.4896297; http://scitation.aip.org/limit_exceeded.html
AIP Publishing
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