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Characterization of a HeLa Cell Factor Which Negatively Regulates Transcriptional Activation in Vitro by Transcriptional Enhancer Factor-1 (TEF-1) (∗)

Journal of Biological Chemistry, ISSN: 0021-9258, Vol: 270, Issue: 8, Page: 3631-3637
1995
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Article Description

A novel negatively acting factor has been identified and partially purified from HeLa and BJA-B cell extracts by chromatographic fractionation. Addition of this factor to HeLa cell extracts or to a reconstituted HeLa cell transcription system repressed transcriptional activation by a chimeric activator, GAL-TEF-1, containing the activation function of transcriptional enhancer factor-1 (TEF-1). In contrast, this factor did not repress transactivation by the chimeric GAL-VP16 activator. Repression of transactivation by GAL-TEF-1 could be alleviated by the addition of immunopurified HeLa cell TFIID, but not by increased quantities of GAL-TEF-1. These observations suggest that this negatively acting factor represses transactivation by interfering with the function of, or competing for, the TATA-binding protein-associated coactivators which mediate the activity TEF-1.

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