Analysis of a Gene Encoding Two Glycine Transporter Variants Reveals Alternative Promoter Usage and a Novel Gene Structure *
Journal of Biological Chemistry, ISSN: 0021-9258, Vol: 273, Issue: 44, Page: 29077-29085
1998
- 60Citations
- 16Captures
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Metrics Details
- Citations60
- Citation Indexes60
- 60
- CrossRef48
- Captures16
- Readers16
- 16
Article Description
The rat GLYT-1 gene encodes two glycine transporter variants, GLYT-1a and GLYT-1b, that differ in NH 2 termini and 5′-noncoding regions as well as in tissue distribution. The GLYT-1 gene contains 15 exons, with the first two specific for GLYT-1a and the third specific for GLYT-1b. By combining RNase protection and rapid amplification of cDNA ends analysis, we have determined transcription start sites for GLYT-1a and GLYT-1b. By using a functional luciferase reporter assay, we demonstrate that distinct promoters regulate the expression of these transporters in several cell lines. Serially truncated GLYT-1b promoter constructs reveal a basal promoter within 304 base pairs of the transcription start site, possible negative regulatory elements between −304 and −1310, and additional positive regulatory elements between −1310 and −5264. The GLYT-1 gene contains three sets of dinucleotide repeats, two AC repeats, and one TG repeat which may form stem-loop structures to either facilitate or interfere with transcription of one of the transporter isoforms. The potential use of dinucleotide repeats in this manner would represent a novel mechanism for gene splicing. The use of distinct promoters for GLYT-1a and GLYT-1b suggests that these transporters have unique regulatory requirements that may reflect the differential tissue-specific expression patterns in white matter (GLYT-1b) and gray matter (GLYT-1a).
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0021925819594947; http://dx.doi.org/10.1074/jbc.273.44.29077; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0032582785&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/9786914; https://linkinghub.elsevier.com/retrieve/pii/S0021925819594947; https://dx.doi.org/10.1074/jbc.273.44.29077
Elsevier BV
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