Overexpression of Frequenin, a Modulator of Phosphatidylinositol 4-Kinase, Inhibits Biosynthetic Delivery of an Apical Protein in Polarized Madin-Darby Canine Kidney Cells *
Journal of Biological Chemistry, ISSN: 0021-9258, Vol: 275, Issue: 32, Page: 24341-24347
2000
- 65Citations
- 22Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations65
- Citation Indexes65
- 65
- CrossRef57
- Captures22
- Readers22
- 22
Article Description
Polyphosphoinositides regulate numerous steps in membrane transport. The levels of individual phosphatidylinositols are controlled by specific lipid kinases, whose activities and localization are in turn regulated by a variety of effectors. Here we have examined the effect of overexpression of frequenin, a modulator of phosphatidylinositol 4-kinase activity, on biosynthetic and postendocytic traffic in polarized Madin-Darby canine kidney cells. Endogenous frequenin was identified in these cells by polymerase chain reaction, Western blotting, and indirect immunofluorescence. Adenoviral-mediated overexpression of frequenin had no effect on early Golgi transport of membrane proteins, as assessed by acquisition of resistance to endoglycosidase H. However, delivery of newly synthesized influenza hemagglutinin from the trans -Golgi network to the apical cell surface was severely inhibited in cells overexpressing frequenin, whereas basolateral delivery of the polymeric immunoglobulin receptor was unaffected. Overexpression of frequenin did not affect postendocytic trafficking steps including apical and basolateral recycling and basal-to-apical transcytosis. We conclude that frequenin, and by inference, phosphatidylinositol 4-kinase, plays an important and selective role in apical delivery in polarized cells.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0021925819621770; http://dx.doi.org/10.1074/jbc.m000671200; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0034637512&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/10825156; https://linkinghub.elsevier.com/retrieve/pii/S0021925819621770; http://www.jbc.org/lookup/doi/10.1074/jbc.M000671200; https://syndication.highwire.org/content/doi/10.1074/jbc.M000671200; https://dx.doi.org/10.1074/jbc.m000671200
Elsevier BV
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