The Tyrosine Kinase Hck Is an Inhibitor of HIV-1 Replication Counteracted by the Viral Vif Protein *
Journal of Biological Chemistry, ISSN: 0021-9258, Vol: 276, Issue: 20, Page: 16885-16893
2001
- 58Citations
- 37Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations58
- Citation Indexes58
- 58
- CrossRef48
- Captures37
- Readers37
- 37
Article Description
The virus infectivity factor (Vif) protein facilitates the replication of human immunodeficiency virus type 1 (HIV-1) in primary lymphocytes and macrophages. Its action is strongly dependent on the cellular environment, and it has been proposed that the Vif protein counteracts cellular activities that would otherwise limit HIV-1 replication. Using a glutathione S -transferase pull-down assay, we identified that Vif binds specifically to the Src homology 3 domain of Hck, a tyrosine kinase from the Src family. The interaction between Vif and the full-length Hck was further assessed by co-precipitation assays in vitro and in human cells. The Vif protein repressed the kinase activity of Hck and was not itself a substrate for Hck phosphorylation. Within one single replication cycle of HIV-1, Hck was able to inhibit the production and the infectivity of vif -deleted virus but not that of wild-type virus. Accordingly, HIV-1 vif − replication was delayed in Jurkat T cell clones stably expressing Hck. Our data demonstrate that Hck controls negatively HIV-1 replication and that this inhibition is suppressed by the expression of Vif. Hck, which is present in monocyte-macrophage cells, represents the first identified cellular inhibitor of HIV-1 replication overcome by Vif.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0021925819318599; http://dx.doi.org/10.1074/jbc.m009076200; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0035907306&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/11278465; https://linkinghub.elsevier.com/retrieve/pii/S0021925819318599; http://www.jbc.org/lookup/doi/10.1074/jbc.M009076200; https://syndication.highwire.org/content/doi/10.1074/jbc.M009076200; https://dx.doi.org/10.1074/jbc.m009076200
Elsevier BV
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