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RecA Protein Filaments Disassemble in the 5′ to 3′ Direction on Single-stranded DNA *

Journal of Biological Chemistry, ISSN: 0021-9258, Vol: 276, Issue: 49, Page: 45740-45743
2001
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Article Description

RecA protein forms filaments on both single- and double-stranded DNA. Several studies confirm that filament extension occurs in the 5′ to 3′ direction on single-stranded DNA. These filaments also disassemble in an end-dependent fashion, and several indirect observations suggest that the disassembly occurs on the end opposite to that at which assembly occurs. By labeling the 5′ end of single-stranded DNA with a segment of duplex DNA, we demonstrate unambiguously that RecA filaments disassemble uniquely in the 5′ to 3′ direction.

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